2WMC
Crystal structure of eukaryotic initiation factor 4E from Pisum sativum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-30 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.612, 136.320, 74.415 |
| Unit cell angles | 90.00, 92.65, 90.00 |
Refinement procedure
| Resolution | 136.083 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.180 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2idv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.481 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.930 | 2.220 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 72233 | |
| <I/σ(I)> | 14.64 | 5.39 |
| Completeness [%] | 97.4 | 95.4 |
| Redundancy | 3.73 | 3.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 18% (W/V) PEG 3350 IN 100 MM HEPES PH 7.0 AT 293 K |
