2WKY
Crystal structure of the ligand-binding core of GluR5 in complex with the agonist 4-AHCP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-06 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.310, 71.310, 234.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.540 - 2.200 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pbw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 31679 | |
| <I/σ(I)> | 12.6 | 2.6 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18 % PEG4000 0.3 M LI2SO4 0.1 M TRIS-HCL PH 7.5 |
