2WKR
Structure of a photoactivatable Rac1 containing the Lov2 C450M Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-11 |
| Detector | MARRESEARCH MX-225 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.390, 112.390, 69.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.601 - 2.200 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1MH1 2vou |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.3) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.110 | 0.500 |
| Number of reflections | 25771 | |
| <I/σ(I)> | 11.7 | 2.6 |
| Completeness [%] | 99.5 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18 % (V/V) PEG 600 |
