2WKQ
Structure of a photoactivatable Rac1 containing the Lov2 C450A Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-26 |
| Detector | MARRESEARCH MX-225 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.638, 112.638, 69.306 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.887 - 1.600 |
| R-factor | 0.1695 |
| Rwork | 0.169 |
| R-free | 0.18650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1MH1 2vou |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.311 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.3) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.450 |
| Number of reflections | 66895 | |
| <I/σ(I)> | 26.5 | 5.8 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 15.4 | 12.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM POTASSIUM CHLORIDE, 5% (W/V) PEG 4000 |
