2WKO
Structure of metal loaded Pathogenic SOD1 Mutant G93A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.488, 47.157, 56.356 |
| Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
| Resolution | 56.800 - 1.970 |
| R-factor | 0.17666 |
| Rwork | 0.174 |
| R-free | 0.23072 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9v |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.520 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.580 | 2.030 |
| High resolution limit [Å] | 1.970 | 1.960 |
| Rmerge | 0.070 | 0.200 |
| Number of reflections | 19287 | |
| <I/σ(I)> | 13 | 2.4 |
| Completeness [%] | 93.6 | 87.9 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 20% P3350, 0.2 M AMMONIUM IODIDE, pH 6.5 |
