2WJY
Crystal structure of the complex between human nonsense mediated decay factors UPF1 and UPF2 Orthorhombic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-25 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 64.910, 129.140, 311.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.520 - 2.500 |
| R-factor | 0.19777 |
| Rwork | 0.196 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2GK7 2iyk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.386 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.770 |
| Number of reflections | 45136 | |
| <I/σ(I)> | 11.2 | 1.9 |
| Completeness [%] | 98.4 | 98 |
| Redundancy | 3.71 | 3.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 1.6M AMMONIUM SULPHATE, 100 MM MES PH 6, 10% DIOXANE |
