2WJV
Crystal structure of the complex between human nonsense mediated decay factors UPF1 and UPF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-23 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.410, 97.290, 124.600 |
| Unit cell angles | 90.00, 102.38, 90.00 |
Refinement procedure
| Resolution | 48.790 - 2.850 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2GK6 AND 2IYK |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.228 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0038) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.090 | 0.760 |
| Number of reflections | 49227 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 97.3 | 97.1 |
| Redundancy | 3.8 | 3.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 1.5-1.6M AMMONIUM SULPHATE, 100 MM MES PH 6.3-6.5 AND 2% V/V GLYCEROL |
