2WII
Complement C3b in complex with factor H domains 1-4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-12-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 223.489, 84.946, 128.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.483 - 2.700 |
| R-factor | 0.2184 |
| Rwork | 0.217 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a74 PDB ENTRY 2I07 PDB ENTRY 1QUB PDB ENTRY 1H03 PDB ENTRY 2RLP |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.446 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 0.610 |
| Number of reflections | 67945 | |
| <I/σ(I)> | 8.3 | 1.9 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 7.0% (W/V) PEG 3,350, 70 MM AMMONIUM ACETATE, PH 7.1 |
