2WEI
Crystal structure of the kinase domain of Cryptosporidium parvum calcium dependent protein kinase in complex with 3-MB-PP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-01 |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.900, 68.900, 130.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.500 - 1.650 |
R-factor | 0.19716 |
Rwork | 0.196 |
R-free | 0.22876 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dfa |
RMSD bond length | 0.014 |
RMSD bond angle | 1.410 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.620 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.110 | 0.700 |
Number of reflections | 38607 | |
<I/σ(I)> | 10.2 | 2.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.3 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 25% PEG 3350, 0.1M BIS-TRIS PH 5.5 |