2WDA
The X-ray structure of the Streptomyces coelicolor A3 Chondroitin AC Lyase in Complex with Chondroitin sulphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-07 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 140.508, 140.508, 100.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.680 - 2.300 |
| R-factor | 0.151 |
| Rwork | 0.149 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wco |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.953 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.300 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.390 |
| Number of reflections | 45361 | |
| <I/σ(I)> | 7.6 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 M NA FORMATE,20 % GLYCEROL AS CRYOPROTECTANT |
