2WDA

The X-ray structure of the Streptomyces coelicolor A3 Chondroitin AC Lyase in Complex with Chondroitin sulphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	PUTATIVE SECRETED LYASE	polymer	765	83022.2	1	UniProt (O86516) Pfam (PF08124) Pfam (PF02278) Pfam (PF02884)	STREPTOMYCES VIOLACEORUBER	PL8
2	B (B)	4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-4-O-sulfo-beta-D-galactopyranose	branched		459.4	1	In PDB GlyTouCan (G76516UK)
3	C, D, E, F, G (A)	FORMIC ACID	non-polymer		46.0	5	Chemie (FMT)
4	H, I, J (A)	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
5	K, L, M, N, O... (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	9	Chemie (PEG)
6	T (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	U (A)	water	water		18.0	499	Chemie (HOH)

Sequence modifications

A: 22 - 765 (UniProt: O86516)

PDB	External Database	Details
Met 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Ser 11	-	expression tag
Ser 12	-	expression tag
Gly 13	-	expression tag
Leu 14	-	expression tag
Val 15	-	expression tag
Pro 16	-	expression tag
Arg 17	-	expression tag
Gly 18	-	expression tag
Ser 19	-	expression tag
His 20	-	expression tag
Met 21	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	83022.2
Branched	Number of molecules	1
	Total formula weight	459.4
Non-Polymers*	Number of molecules	18
	Total formula weight	1485.8
All*	Total formula weight	84967.4

*Water molecules are not included.