2WAQ

The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	193.501, 212.620, 128.998
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.780 - 3.350
R-factor	0.274
Rwork	0.271
R-free	0.34100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1EN0
RMSD bond length	0.006
RMSD bond angle	1.027
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.800
High resolution limit [Å]	3.350	3.350
Number of reflections	59401
<I/σ(I)>	8.5	1.7
Completeness [%]	77.0	44.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		7MG/ML ENZYME, 150MM KCL, 100MM SRCL2, 1MM ZNCL2, 100MM CACODYLATE PH 6.5, 12% PEG MME 5K, 5% GLYCEROL