2WAF
PENICILLIN-BINDING PROTEIN 2B (PBP-2B) FROM STREPTOCOCCUS PNEUMONIAE (STRAIN R6)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-09 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.897, 51.593, 121.440 |
| Unit cell angles | 90.00, 112.38, 90.00 |
Refinement procedure
| Resolution | 47.250 - 3.290 |
| R-factor | 0.24 |
| Rwork | 0.237 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wad |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.287 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0067) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.250 | 3.490 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Number of reflections | 14291 | |
| <I/σ(I)> | 11.2 | 2.82 |
| Completeness [%] | 82.5 | 54.9 |
| Redundancy | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 1.4M (NH4)2SO4, pH 8 |
