2WA7
Structure of the M202V mutant of human filamin b actin binding domain at 1.85 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-22 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.079, 69.828, 87.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.830 - 1.850 |
| R-factor | 0.19358 |
| Rwork | 0.191 |
| R-free | 0.23314 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wa5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.148 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.830 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.040 | 0.520 |
| Number of reflections | 23537 | |
| <I/σ(I)> | 25.8 | 2.2 |
| Completeness [%] | 95.5 | 79.5 |
| Redundancy | 4.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 20% PEG 8000, 0.2 M MAGNESIUM ACETATE AND 0.1 M SODIUM CACODYLATE PH 6.5. |
