2W99

Crystal Structure of CDK4 in complex with a D-type cyclin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-13
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	57.023, 64.684, 188.747
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.490 - 2.800
R-factor	0.216
Rwork	0.213
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1blx
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER-TNT (2.1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.500	2.910
High resolution limit [Å]	2.800	2.800
Rmerge	0.090	0.540
Number of reflections	17640
<I/σ(I)>	6	1.5
Completeness [%]	98.6	94.8
Redundancy	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1