2W8M
Structure of D212, a nuclease from a fusselovirus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-21 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.85, 2.06 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.477, 81.971, 60.160 |
| Unit cell angles | 90.00, 115.40, 90.00 |
Refinement procedure
| Resolution | 29.100 - 2.400 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24500 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.698 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.030 | 0.330 |
| Number of reflections | 17143 | |
| <I/σ(I)> | 32 | 4.3 |
| Completeness [%] | 92.9 | 95.7 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES (PH 7.5), 0.05 M K2HPO4, 10% (W/V) PEG 8000 WITH 25% GYLCEROL AS CRYOPROTECTANT | ||
| 1 | 7.5 | 0.1 M HEPES (PH 7.5), 0.05 M K2HPO4, 10% (W/V) PEG 8000 WITH 25% GYLCEROL AS CRYOPROTECTANT |
