2W81
Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-12 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 185.520, 52.210, 128.780 |
| Unit cell angles | 90.00, 118.19, 90.00 |
Refinement procedure
| Resolution | 38.810 - 2.350 |
| R-factor | 0.275 |
| Rwork | 0.274 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uwn |
| Data reduction software | xia2 (WITH XDS) |
| Data scaling software | xia2 (WITH SCALA) |
| Phasing software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.050 | 0.320 |
| Number of reflections | 43159 | |
| <I/σ(I)> | 20.6 | 4.5 |
| Completeness [%] | 94.3 | 95.5 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 20% PEG 6000, 0.1M BICINE PH 9.0 |
