2W66
BtGH84 in complex with HQ602
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.199, 93.465, 99.010 |
| Unit cell angles | 104.16, 94.01, 103.02 |
Refinement procedure
| Resolution | 95.350 - 2.270 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vvn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.596 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.350 | 2.390 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.080 | 0.430 |
| Number of reflections | 76272 | |
| <I/σ(I)> | 9.7 | 2.6 |
| Completeness [%] | 96.0 | 94.7 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.3M NH4AC 10% GLYCEROL 15% PEG3350 0.1M MES PH6 |
