2W36
Structures of endonuclease V with DNA reveal initiation of deaminated adenine repair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.979, 132.239, 191.616 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.350 |
| Number of reflections | 32087 | |
| <I/σ(I)> | 69 | 5.9 |
| Completeness [%] | 87.6 | 62 |
| Redundancy | 13.3 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
