2W2W
PLCg2 Split Pleckstrin Homology (PH) Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-13 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.216, 106.017, 194.097 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 97.050 - 2.800 |
R-factor | 0.306 |
Rwork | 0.303 |
R-free | 0.35200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1upq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.226 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 3.480 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.090 | 0.560 |
Number of reflections | 28821 | |
<I/σ(I)> | 7.1 | 1.4 |
Completeness [%] | 98.4 | 95.2 |
Redundancy | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | PLCSPPH(Y495F) WAS CRYSTALLIZED USING A PROTEIN CONCENTRATION OF 36 MG/ML WITH PRECIPITANT (20% PEG3350, 100 MM BIS-TRIS PROPANE PH 7.5) AT A CONSTANT TEMPERATURE OF 20C |