2W2J

Crystal structure of the human carbonic anhydrase related protein VIII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	287
Detector technology	CCD
Collection date	2008-10-04
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	44.267, 73.854, 87.908
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.860 - 1.600
R-factor	0.201
Rwork	0.199
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2q1b
RMSD bond length	0.007
RMSD bond angle	1.123
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.990	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.090	0.580
Number of reflections	38029
<I/σ(I)>	11.5	1.8
Completeness [%]	98.1	87.2
Redundancy	6.3	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.3		20 MM NH4CL, 20.0% PEG 6K, 10.0% GLYCEROL, pH 6.3