2W22
Activation Mechanism of Bacterial Thermoalkalophilic Lipases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-21 |
| Detector | ADSC CCD |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 73.070, 128.080, 127.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.180 - 2.200 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ji3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.750 | 2.360 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.140 | 0.480 |
| Number of reflections | 37406 | |
| <I/σ(I)> | 8.9 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | SODIUM CITRATE 0.05 M, PH 5.6, MPD 13 % AND AMMONIUM ACETATE 0.2 M |
