2W19
Non-covalent complex between dahp synthase and chorismate mutase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 204.018, 204.018, 66.513 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.820 - 2.150 |
| R-factor | 0.17971 |
| Rwork | 0.178 |
| R-free | 0.21115 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b7o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.244 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.820 | 2.170 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.110 | 0.530 |
| Number of reflections | 83270 | |
| <I/σ(I)> | 14.26 | 1.93 |
| Completeness [%] | 96.6 | 81 |
| Redundancy | 6.84 | 3.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 0.9M AMMONIUM SULFATE, 0.1M TRIS PH 7.9, 5% GLYCEROL |
