2W0X
FACTOR INHIBITING HIF-1 ALPHA WITH PYRIDINE 2,4 DICARBOXYLIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.1 |
| Synchrotron site | SRS |
| Beamline | PX14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.471, 86.471, 147.237 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.670 - 2.120 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h2n |
| RMSD bond length | 0.000 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.200 | 2.240 |
| High resolution limit [Å] | 2.130 | 2.120 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 32255 | |
| <I/σ(I)> | 16.9 | 1.8 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 8.2 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.6M AMMONIUM SULFATE, 0.1M HEPES PH 7.5, 5% PEG400, 1MM FESO4, 2MM SUBSTRATE |
