2W0P
Crystal structure of the filamin A repeat 21 complexed with the migfilin peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-17 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.890, 68.480, 85.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.480 - 1.900 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2brq CHAIN A |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.789 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.480 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 0.740 |
| Number of reflections | 17625 | |
| <I/σ(I)> | 13.5 | 2.35 |
| Completeness [%] | 99.9 | 97.7 |
| Redundancy | 2.54 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.7M (NH4)2SO4, 5% 2-PROPANOL |
