2VZX
Structural and spectroscopic characterization of photoconverting fluorescent protein Dendra2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 |
Unit cell lengths | 71.260, 76.850, 92.500 |
Unit cell angles | 90.05, 108.17, 106.58 |
Refinement procedure
Resolution | 33.500 - 2.000 |
R-factor | 0.21517 |
Rwork | 0.212 |
R-free | 0.26890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zux |
RMSD bond length | 0.009 |
RMSD bond angle | 1.415 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0038) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.500 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.050 | 0.170 |
Number of reflections | 113215 | |
<I/σ(I)> | 12.15 | 4.42 |
Completeness [%] | 94.1 | 94.5 |
Redundancy | 1.95 | 1.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.8 | 32% PEG 3350, 0.1M MAGNESIUM NITRATE, PH 8.8 |