2VXU
Crystal structure of murine reference antibody 125-2H Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.061, 92.490, 137.645 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.420 - 2.360 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fns |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.501 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.500 | 2.390 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.050 | 0.240 |
| Number of reflections | 41229 | |
| <I/σ(I)> | 18.9 | 4.9 |
| Completeness [%] | 94.0 | 25.2 |
| Redundancy | 5.2 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 277 | FAB (13 MG/ML, 2 MICROLITERS [UL]) WAS MIXED WITH 2 UL OF RESERVOIR (10% POLYETHYLENEGLYCOL (PEG) 6000, 100 MM HEPES, PH 7.5, 5% 2, 4-METHYLPENTANEDIOL) AND SUSPENDED OVER THE RESERVOIR AT 277 K. ROD-LIKE CRYSTALS APPEARED WITHIN ONE DAY. |
