2VXU

Crystal structure of murine reference antibody 125-2H Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-20
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	79.061, 92.490, 137.645
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	17.420 - 2.360
R-factor	0.187
Rwork	0.184
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fns
RMSD bond length	0.014
RMSD bond angle	1.501
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.500	2.390
High resolution limit [Å]	2.330	2.330
Rmerge	0.050	0.240
Number of reflections	41229
<I/σ(I)>	18.9	4.9
Completeness [%]	94.0	25.2
Redundancy	5.2	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	277	FAB (13 MG/ML, 2 MICROLITERS [UL]) WAS MIXED WITH 2 UL OF RESERVOIR (10% POLYETHYLENEGLYCOL (PEG) 6000, 100 MM HEPES, PH 7.5, 5% 2, 4-METHYLPENTANEDIOL) AND SUSPENDED OVER THE RESERVOIR AT 277 K. ROD-LIKE CRYSTALS APPEARED WITHIN ONE DAY.