2VX3
Crystal structure of the human dual specificity tyrosine- phosphorylation-regulated kinase 1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-09 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 264.203, 65.105, 140.282 |
| Unit cell angles | 90.00, 115.44, 90.00 |
Refinement procedure
| Resolution | 26.000 - 2.400 |
| R-factor | 0.186 |
| Rwork | 0.185 |
| R-free | 0.23000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EXE AND 1Z57 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.518 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.350 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 92815 | |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 34% PEG 300, 0.1 M LISO4, TRIS PH 8.5 |
