2VWE
Crystal Structure of Vascular Endothelial Growth Factor-B in Complex with a Neutralizing Antibody Fab Fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.200, 94.300, 91.300 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 19.780 - 3.400 |
| R-factor | 0.286 |
| Rwork | 0.262 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CFN AND 2C7W |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.610 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.120 | 0.260 |
| Number of reflections | 17540 | |
| <I/σ(I)> | 7.4 | 2.7 |
| Completeness [%] | 93.0 | 72.4 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M HEPES PH 7.0 15% GLYCEROL 8% PEG 8000 |
