2VUM
Alpha-amanitin inhibited complete RNA polymerase II elongation complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-06-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 220.619, 394.234, 283.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| R-factor | 0.2547 |
| Rwork | 0.255 |
| R-free | 0.28760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y1w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.533 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.130 | 0.470 |
| Number of reflections | 328642 | |
| <I/σ(I)> | 8.5 | 3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 200 MM AMMONIUM ACETATE, 300 MM SODIUM ACETATE, 50 MM HEPES PH 7.0, 4-7% PEG 6000, 5 MM TCEP |
