2VSZ

Crystal Structure of the ELMO1 PH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-02-20
Detector	ADSC CCD
Spacegroup name	P 61 2 2
Unit cell lengths	166.016, 166.016, 81.704
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	144.340 - 2.300
R-factor	0.209
Rwork	0.208
R-free	0.23900
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.021
RMSD bond angle	1.944
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	SHELXCDE
Refinement software	REFMAC (5.4.0066)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.060	0.230
Number of reflections	29960
<I/σ(I)>	22.7	8.3
Completeness [%]	99.9	100
Redundancy	6.3	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.75		2.1 M SODIUM MALONATE [PH 6.75]