2VRT
Crystal Structure of E. coli RNase E possessing M1 RNA fragments - Catalytic Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-19 |
| Detector | QUANTUM |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.894, 121.252, 242.228 |
| Unit cell angles | 90.00, 99.47, 90.00 |
Refinement procedure
| Resolution | 24.980 - 3.500 |
| R-factor | 0.321 |
| Rwork | 0.317 |
| R-free | 0.35100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bx2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.134 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.500 | 3.690 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.120 | 0.390 |
| Number of reflections | 33891 | |
| <I/σ(I)> | 9.6 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 10% PEG 4000, 100 MM (NH4)2SO4, AND 100 MM HEPES PH 7.5 |
