2VQZ
Structure of the cap-binding domain of influenza virus polymerase subunit PB2 with bound m7GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-08 |
Detector | ADSC CCD |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 92.200, 94.440, 220.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.530 - 2.300 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.23500 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.013 |
RMSD bond angle | 1.519 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.080 | 0.480 |
Number of reflections | 40785 | |
<I/σ(I)> | 8.59 | 1.79 |
Completeness [%] | 98.4 | 97.3 |
Redundancy | 3.48 | 3.38 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 1 MICROLITRE OF PROTEIN SOLUTION AT 11 MG PER ML IN 50 MM TRIS-HCL (PH 8.0), 200 MM NACL, 2 MM DTT, 5 MM M7GTP WITH AN EQUAL VOLUME OF A SOLUTION CONTAINING 0.1 M CITRIC ACID PH 4.6, 1.6-1.8 M SODIUM FORMATE. |