2VOJ
Ternary complex of M. tuberculosis Rv2780 with NAD and pyruvate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 88.993, 88.993, 373.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 377.960 - 2.600 |
| R-factor | 0.18886 |
| Rwork | 0.187 |
| R-free | 0.22998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.835 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.920 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 46674 | |
| <I/σ(I)> | 30.3 | 7.9 |
| Completeness [%] | 98.0 | 99.2 |
| Redundancy | 7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
