2VOI
Structure of mouse A1 bound to the Bid BH3-domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.737, 80.808, 32.184 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.940 - 2.100 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vof |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.161 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.540 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.550 |
| Number of reflections | 9930 | |
| <I/σ(I)> | 19.1 | 2.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M CITRIC ACID, KOH (PH 4.2), 18% PEG 2000, 0.4 M LICL |
