2VNF

MOLECULAR BASIS OF HISTONE H3K4ME3 RECOGNITION BY ING4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-12
Detector	ADSC CCD
Spacegroup name	P 43
Unit cell lengths	68.516, 68.516, 28.174
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.510 - 1.760
R-factor	0.16
Rwork	0.156
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g6q
RMSD bond length	0.012
RMSD bond angle	1.816
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.510	1.810
High resolution limit [Å]	1.760	1.760
Rmerge	0.080	0.380
Number of reflections	12178
<I/σ(I)>	9.9	1.7
Completeness [%]	96.9	86.8
Redundancy	3.8	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1