2VN9
Crystal Structure of Human Calcium Calmodulin dependent Protein Kinase II delta isoform 1, CAMKD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.276, 68.276, 313.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.030 - 2.300 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v7o |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.425 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.530 |
| Number of reflections | 39106 | |
| <I/σ(I)> | 10.7 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.6 M NAKPO4 HEPES PH 7.5 |
