2VMY
Crystal structure of F351GbsSHMT in complex with Gly and FTHF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.382, 103.483, 61.959 |
Unit cell angles | 90.00, 90.33, 90.00 |
Refinement procedure
Resolution | 20.810 - 2.700 |
R-factor | 0.218 |
Rwork | 0.215 |
R-free | 0.26300 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1kl2 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.985 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.090 | 0.510 |
Number of reflections | 19879 | |
<I/σ(I)> | 10 | 2.4 |
Completeness [%] | 98.7 | 99.8 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 50% MPD, 0.1 M HEPES PH 7.5, 0.2 MM EDTA, 5 MM 2-MERCAPTOETHANOL, 10 MM GLY, PROTEIN INCUBATED WITH 2 MM FTHF |