2VJ3
Human Notch-1 EGFs 11-13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 28.000, 28.000, 281.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 95.400 - 2.600 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1edm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.680 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | TNT (5.6.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.250 | 2.710 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.060 | 0.150 |
| Number of reflections | 4321 | |
| <I/σ(I)> | 25.2 | 5.3 |
| Completeness [%] | 93.3 | 93.3 |
| Redundancy | 8.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.7 | 30% (W/V) PEG-5000-MME, 100MM SODIUM ACETATE, PH5.7 |
