2VGQ

Crystal Structure of Human IPS-1 CARD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 41 21 2
Unit cell lengths	99.260, 99.260, 163.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.240 - 2.100
R-factor	0.183
Rwork	0.181
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.507
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.700	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.070	0.360
Number of reflections	48262
<I/σ(I)>	16.1	4.4
Completeness [%]	99.8	99.9
Redundancy	7.1	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1