2VFE
Crystal structure of F96S mutant of Plasmodium falciparum triosephosphate isomerase with 3- phosphoglycerate bound at the dimer interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.843, 50.872, 87.067 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 43.900 - 2.200 |
| R-factor | 0.22115 |
| Rwork | 0.218 |
| R-free | 0.27563 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o5x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.893 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 23622 | |
| <I/σ(I)> | 10.6 | 1.98 |
| Completeness [%] | 97.6 | 87.3 |
| Redundancy | 2.95 | 2.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M SODIUM ACETATE PH 4.0 -5.5, PEG 1450 8-24% |
