2VF6

Human FDPS synthase in complex with minodronate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	298
Detector technology	CCD
Collection date	2005-05-03
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	111.207, 111.207, 69.059
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.570 - 2.100
R-factor	0.202
Rwork	0.199
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rah
RMSD bond length	0.010
RMSD bond angle	1.117
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.030	0.310
Number of reflections	25378
<I/σ(I)>	22.22	3.57
Completeness [%]	98.1	96.6
Redundancy	7.4	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	298	0.2M AMMONIUM SULFATE, 15% ISOPROPANOL, 15% ETHYLENE GLYCOL, 0.1M SODIUM ACETATE PH 4.5, VAPOR DIFFUSION, TEMPERATURE 298K