2VCY
Crystal Structure of 2-Enoyl Thioester Reductase of Human FAS II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 104.620, 104.620, 146.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.790 - 2.410 |
| R-factor | 0.2101 |
| Rwork | 0.207 |
| R-free | 0.26740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1guf |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.790 | 2.540 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.050 | 0.350 |
| Number of reflections | 32130 | |
| <I/σ(I)> | 14.7 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.2 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 2M AMMONIUM SULFATE, 100 MM TRIS HCL, PH 8.5 |
