2VBV
Riboflavin kinase Mj0056 from Methanocaldococcus jannaschii in complex with CDP and FMN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.482, 73.299, 104.907 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.232 |
| Rwork | 0.227 |
| R-free | 0.32300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vbt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.580 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.690 |
| Number of reflections | 12903 | |
| <I/σ(I)> | 8.67 | 2.25 |
| Completeness [%] | 98.6 | 98.5 |
| Redundancy | 5.5 | 5.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% W/V PEG 8000, 200 MM SODIUM IODIDE |
