2VBI

Holostructure of pyruvate decarboxylase from Acetobacter pasteurianus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-19
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	179.619, 162.434, 169.331
Unit cell angles	90.00, 102.71, 90.00

Refinement procedure

Resolution	30.000 - 2.750
R-factor	0.225
Rwork	0.224
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zpd
RMSD bond length	0.007
RMSD bond angle	1.095
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.440	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.080	0.430
Number of reflections	119238
<I/σ(I)>	10.8	2
Completeness [%]	97.2	98.5
Redundancy	2.4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1