2V8N
Wild-type Structure of Lactose Permease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.370, 127.400, 188.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.600 |
| R-factor | 0.294 |
| Rwork | 0.294 |
| R-free | 0.33300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pv7 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.120 | 0.490 |
| Number of reflections | 24578 | |
| Completeness [%] | 85.8 | 75.6 |
| Redundancy | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8.0 |
