2V83

Crystal structure of RAG2-PHD finger in complex with H3K4me3 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2006-03-06
Detector	RIGAKU-MSC
Spacegroup name	P 1 21 1
Unit cell lengths	54.783, 46.835, 56.963
Unit cell angles	90.00, 101.46, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.1961
Rwork	0.196
R-free	0.23020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PROTEIN MODEL DETERMINED BY SAD
RMSD bond length	0.007
RMSD bond angle	1.504
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.150	0.490
Number of reflections	11048
<I/σ(I)>	8.1	1.8
Completeness [%]	99.4	95.7
Redundancy	3.3	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	10% PEG 5000 MONOMETHYL ETHER, 0.1 M BIS-TRIS PH 6.5 VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K.
1	VAPOR DIFFUSION, HANGING DROP		298	10% PEG 5000 MONOMETHYL ETHER, 0.1 M BIS-TRIS PH 6.5 VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K.