2V79
Crystal Structure of the N-terminal domain of DnaD from Bacillus Subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-05-27 |
| Detector | RIGAKU IMAGE-PLATE |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 78.666, 78.666, 124.558 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.21000 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.126 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.050 | 0.380 |
| Number of reflections | 30662 | |
| <I/σ(I)> | 24.4 | 4 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M SODIUMACETATE, 3.2M SODIUMCHLORIDE |
