2V5P
COMPLEX STRUCTURE OF HUMAN IGF2R DOMAINS 11-13 BOUND TO IGF-II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-13 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.979, 117.312, 116.668 |
| Unit cell angles | 90.00, 123.41, 90.00 |
Refinement procedure
| Resolution | 47.510 - 4.100 |
| R-factor | 0.293 |
| Rwork | 0.291 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v5o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.906 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 4.200 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.290 | 0.550 |
| Number of reflections | 14662 | |
| <I/σ(I)> | 5.4 | 2.5 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 0.1 M TRIS-HCL PH 9.0, 15% (V/V) MPD |
