2V5E
The structure of the GDNF:Coreceptor complex: Insights into RET signalling and heparin binding.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 190 |
| Detector technology | CCD |
| Collection date | 2006-09-06 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.900, 75.700, 105.500 |
| Unit cell angles | 90.00, 91.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.187 |
| Rwork | 0.184 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1AGQ CHAIN A 2GH0 CHAIN A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.206 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.450 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.080 | 0.290 |
| Number of reflections | 19071 | |
| <I/σ(I)> | 16 | 4.29 |
| Completeness [%] | 98.2 | 87 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM HEPES PH7.5, 10% PEG-8K, 8% ETHYLENE GLYCOL |
